The metabolism of the insecticide carbaryl (1-naphthyl N-methylcarbamate) by fat body of the blowfly larva Calliphora erythrocephala.
نویسندگان
چکیده
1. Carbaryl is metabolized more rapidly by fat body of the blowfly larva than by gut, muscle, cuticle or haemolymph. 2. Metabolism of carbaryl by the fat body is affected by the age of the larva, the pH of the incubation medium, and the concentration of magnesium chloride in the incubation medium. 3. Chloramphenicol, 2,4-dinitrophenol and 5-dimethylamino-6-nitro-1,3-benzodioxole (a carbaryl synergist) inhibit carbaryl metabolism by the fat body. 4. Subcellular fractionation of the fat body indicates that the pellet sedimenting at 30000g is the most reactive with carbaryl. 5. Probable metabolites of carbaryl formed by the fat body include the 4- and 5-hydroxy derivatives, and, possibly, the N-hydroxymethyl and 5,6-dihydrodihydroxy derivatives.
منابع مشابه
Purification and characterization of the N-methylcarbamate hydrolase from Pseudomonas strain CRL-OK.
A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N,N-dipropylthiocarbamate, or the d...
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Electron diffraction techniques have been extensively used for the direct crystallographic examination of thin films of fatty materials, but they have hitherto not been applied to the study of natural membranes. These techniques provide more information than diffraction of X-rays about the atomic and molecular organization of surface structures. X-ray diffraction methods have been used successf...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 112 2 شماره
صفحات -
تاریخ انتشار 1969